(A,B) DHE fluorescence probe detection of ROS (n=3), significant differences (P<0.05) were observed among a–d. (C) MDA in BMSCs content detection, significant differences (P<0.05) were observed among a–c. (D) Analysis of CAT activity in BMSCs, significant differences (P<0.05) were observed among a–d. (E) Analysis of Mn-SOD activity in BMSCs, significant differences (P<0.05) were observed among a–c. (F) Detection of the ratio of GSH/GSSH in BMSCs, significant differences (P<0.05) were observed among a–c. (G,H) Detection of β-gal activity by β-gal staining (n=4), significant differences (P<0.05) were observed among a–c. (I,J) TUNEL/DAPI staining method for detecting apoptosis (n=4), significant differences (P<0.05) were observed among a–c. All the experiments were repeated at least thrice (n≥3). Data were presented as means ± SD. P<0.05 by one-way ANOVA with Tukey’s post hoc test (three or more groups).