MiR-495-3p down-regulates GRP78 expression via the p-AKT/mTOR signaling pathway
(A) According to the TCGA database, MiR-495 expression was higher in normal breast tissues than in cancer tissues. (B) q-PCR results showed that MDA-MB-231R cells expressed lower miR-495-3p levels than MDA-MB-231 cells. (C) Transfecting miR-495-3p inhibitors in MDA-MB-231 cells decreased miR-495-3p expression, while transfecting miR-495-3p mimics increased it. (D) Immunofluorescence staining results showing that down-regulating miR-495-3p increased GRP78 (green staining) expression in MDA-MB-231 cells, and overexpressing miR-495-3p down-regulated GRP78 expression in MDA-MB-231R cells. Blue: nuclei (DAPI staining). (E) Western blot showing that transfecting miR-495-3p inhibitors in MDA-MB-231 cells increased the GRP78, p-AKT, and mTOR levels, and transfecting miR-495-3p mimics in MDA-MB-231R cells decreased them. Left panel: Western blot; right panel: quantification. ***P<0.001.