Hsa_circ_0076931/miR-6760-3p/CCBE1 axis is predicted to play important roles in the progression of glioma
(A) circRNA/miRNA/mRNA ceRNA network analysis. (B) The level of CCBE1 in H4 and U118-MG cells was detected by qRT-PCR after transfection with hsa_circ_0076931. (C) The level of CCBE1 in H4 and U118-MG cells was detected by Western blot after transfection with hsa_circ_0076931. (D) Quantification of CCBE1 protein is shown. (E) The level of miR-6067-3p in H4 and U118-MG cells was detected by qRT-PCR after transfection with hsa_circ_0076931. (F) Schematic of the binding site between hsa_circ_0076931 and miR-6067-3p, and the luciferase activity of WT hsa_circ_0076931 3′ UTR or mutant hsa_circ_0076931 3′ UTR after transfection with miR-6067-3p mimics in 293T cells. (G) Schematic of the binding site between miR-6067-3p and CCBE1. (H) AGO2-RIP assay was used to analyze the expression of hsa_circ_0076931, miR-6760-3p, and CCBE1 in U118-MG cells. (I) After interference with hsa_circ_0076931, the expression of CCBE1 was tested by AGO2-RIP assay in U118-MG cells; *P<0.05, **P<0.01, ***P<0.001.