MiR-34a-3p attenuated the increase in DUSP1 expression in PDGF-BB-induced PASMCs
(A) RT-qPCR indicated the level of DUSP1 in PASMCs following transfection with DUSP1 plasmid. (B) RT-qPCR indicated the level of miR-34a-3p in PASMCs after PDGF-BB induction and nucleic acid transfection. (C,D) Western blot indicated the level of DUSP1 in PASMCs after PDGF-BB induction and nucleic acid transfection in PASMCs. (E) CCK-8 indicated cell viability of PASMCs after PDGF-BB induction and nucleic acid transfection. U6 was used as the internal control for miR-34a-3p. GAPDH was used as the internal control for DUSP1. Control, without any treatment; NC, negative control for DUSP1 plasmid; M, miR-34s-3p mimic; M, miR-34a-3p mimic. ***P<0.001 vs. Control; ∧P<0.05, ∧∧P<0.01, ∧∧∧P<0.001 vs. PDGF-BB+MC+NC; ##P<0.01, ###P<0.001 vs. PDGF-BB+MC+DUSP1; ‡P<0.05, ‡‡P<0.01 vs. PDGF-BB+M+NC; §§§P<0.001 vs. NC. Data are presented as mean ± SD (n=3 in each group).