MiR-34a-3p suppressed DUSP1 expression by directly binding to its 3′-UTR
(A) Prediction of miR-34a-3p target genes was conducted through TargetScan, miRDB, miRWalk and RNAcentral websites. (B) RT-qPCR indicated the levels of DUSP1, ATXN7L3B, OLA1, SLC22A5 and TMEM107 in PASMCs. (C–E) The binding relationship between miR-34a-3p and DUSP1 was predicted by TargetScan, and verified by dual-luciferase reporter (D) and RNA pull-down assays (E). PASMCs, pulmonary artery smooth muscle cells; 3′-UTR, 3′-untranslated region; RT-qPCR, quantitative reverse transcription polymerase chain reaction; M, miR-34a-3p mimic; Bio-NC-probe, biotin-labeled RNA. ∧P<0.05, ∧∧P<0.01, ∧∧∧P<0.001 vs. MC; ***P<0.001 vs. Bio-NC-probe. Data are presented as mean ± SD (with n=3 in each group).