Portland Press

Figure 4

(A and B) The mRNA (A) and protein expression (B) of AT1R were determined by qt-PCR and immunoblotting in the 12-week-old PM2.5 offspring. AT1R mRNA level was normalized using GAPDH. The protein expression of AT1R was normalized using tubulin expression (A and B, two-tailed, unpaired t test, *P < 0.05 vs. control, n = 6). (C) Effect of losartan (20 mg/kg/d, 4 weeks) on the systolic blood pressure (SBP) in the PM2.5 offspring (two-way ANOVA with Tukey’s post hoc test, *P < 0.05 vs. P- offspring +losartan, n = 8; #P < 0.05 vs. C-offspring, n = 8). (D and E) Effect of losartan (20 mg/kg/d, 4 weeks) on the 24 h urine volume (D) and sodium excretion (E) in the PM2.5 offspring. (D and E, two-way ANOVA with Tukey’s post hoc test, *P < 0.05 vs. C-offspring, n = 8; #P < 0.05 vs. P-offspring, n = 8). (F and G) The mRNA (F) and protein expression (G) of GRK4 were determined by qt-PCR and immunoblotting in the 12-week-old PM2.5 offspring. AT1R mRNA level was normalized using GAPDH. The protein expressions of GRK4 were normalized using tubulin expression (F and G, two-tailed, unpaired t test, *P < 0.05 vs. C-offspring, n = 6). Control offspring (C or C-offspring): offspring of vehicle-exposed paternal SD rats; PM2.5 offspring (P or P-offspring): offspring of PM2.5-exposed paternal rats.

AT₁R and GRK4 expression and function in the offspring of PM_2.5-exposed paternal rats

(A and B) The mRNA (A) and protein expression (B) of AT₁R were determined by qt-PCR and immunoblotting in the 12-week-old PM_2.5 offspring. AT₁R mRNA level was normalized using GAPDH. The protein expression of AT₁R was normalized using tubulin expression (A and B, two-tailed, unpaired t test, *P < 0.05 vs. control, n = 6). (C) Effect of losartan (20 mg/kg/d, 4 weeks) on the systolic blood pressure (SBP) in the PM_2.5 offspring (two-way ANOVA with Tukey’s post hoc test, *P < 0.05 vs. P- offspring +losartan, n = 8; ^#P < 0.05 vs. C-offspring, n = 8). (D and E) Effect of losartan (20 mg/kg/d, 4 weeks) on the 24 h urine volume (D) and sodium excretion (E) in the PM_2.5 offspring. (D and E, two-way ANOVA with Tukey’s post hoc test, *P < 0.05 vs. C-offspring, n = 8; ^#P < 0.05 vs. P-offspring, n = 8). (F and G) The mRNA (F) and protein expression (G) of GRK4 were determined by qt-PCR and immunoblotting in the 12-week-old PM_2.5 offspring. AT₁R mRNA level was normalized using GAPDH. The protein expressions of GRK4 were normalized using tubulin expression (F and G, two-tailed, unpaired t test, *P < 0.05 vs. C-offspring, n = 6). Control offspring (C or C-offspring): offspring of vehicle-exposed paternal SD rats; PM_2.5 offspring (P or P-offspring): offspring of PM_2.5-exposed paternal rats.

This Feature Is Available To Subscribers Only