Mycolactone A (MYC-A, top-left) was docked to homology models of human WT Sec61α. Overviews depict Sec61α seen from the cytosol and from the plane of the membrane with the cytosolic side upward (Sec61α, top-right). The most favourable binding poses of mycolactone A in the idle (lower-left), mycolactone-inhibited (lower-middle) and open (lower-right) state-homology models are shown. The mycolactone conformation from cryo-EM structure PDB 6Z3T (orange) is super imposed on the docking result for the inhibited state . For the open state, two different conformations are shown; ‘extended' and ‘ring-like'. TM, transmembrane helix; CL, cytoplasmic loop.