(A) Western blots of xbp1s and Ddit3 and relative quantification analysis. PASMCs were cultured under normoxia, hypoxia with negative control transfection, hypoxia with siRNA transfection to silence xbp1s expression, hypoxia with cotransfection of siRNA to silence xbp1s and plasmid to overexpress Ddit3. (B) Representative image of the EdU staining assay, which was performed to detect the proliferation of PASMCs. (C) Representative image of the Transwell assay, which was performed to detect the migration ability of PASMCs. (original magnification, ×100). (D) Representative images and summary data of Annexin-V FITC/PI double staining. (E) Results of the cell viability assay, which was determined by Cell Counting Kit 8 (CCK-8). (F) Summarized data of EdU staining. (G) Summarized data of migrated cell numbers. (H) Summarized data of apoptosis rates. The early and late apoptosis rates were summed to calculate the total apoptosis rate. Data are represented as the mean ± SD, n=3. ns, no significance, *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.