Proteins that interact with xbp1s
(A) Image showing the protein interaction network focused on xbp1s. Data were predicted by the STRING database. (B) qRT-PCR results showing the relative expression of proteins predicted to interact with xbp1s. Expression was detected in PASMCs cultured under normoxia, hypoxia, and hypoxia with xbp1s silencing. (C) Western blots showing xbp1s, Hsp90b1, and Ddit3 expression levels in PASMCs cultured in the indicated context. N-normoxia, H-hypoxia, Hsi-hypoxia+si-xbp1s. Relative quantification analysis of Hsp90b1 (D), xbp1s (E), and Ddit3 (F) shown in Figure 1(C). (G) Co-IP of Ddit3 with xbp1s. Data are represented as the mean ± SD, n=3. ns, no significance, *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. Abbreviation: Co-IP, co-immunoprecipitation.