(A,B) MDA-MB-231 and MTV/TM-011 cells were seeded in 12-well plates and wounded by 1-ml pipette tip. The cells were incubated with TGF-β (10 ng/ml) or sauchinone (25 μM) for 48 h, separately or in combination. Scale bar = 200 µm. (C) Cells were pretreated with TGF-β (10 ng/ml) for 24 h and then incubated with sauchinone (25 μM) for another 24 h. Proteins were performed by Western blot analysis. (D,E) MTV/TM-011 cells were seeded in Matrigel-coated inserts and incubated with TGF-β (10 ng/ml) or sauchinone (25 μM) for 30 h, separately or in combination, followed by invasion assay. The invaded cells were counted and quantified using ImageJ. Scale bar = 200 µm. Data are presented using triplicate wells per group and statistical significance was determined by one-way ANOVA. *, P < 0.05; **, P < 0.01; ***, P < 0.001; NS, not significant.