(A) Representative Western blot images of SIRT3. (B) Densitometric analysis of SIRT3 protein expression normalized to GAPDH content. (C) Representative IHC images of SIRT3 in different groups. The expression of SIRT3 was significantly reduced in the CKD group. The combination of PE and JPYSF could significantly up-regulate the expression of SIRT3. All images are shown at identical magnification, ×100, scale bar = 100 μm. Data are presented as the means ± SEM, n=4–6 rats per group (***P<0.001 compared with the control group; ^#P<0.05 compared with the CKD group; ns, no significance).