Pharmacological inhibition and genetic knockdown of PEPT1 decrease the growth of PDAC cells in mouse xenografts.
Capan-1 and SU.86.86 cells were subcutaneously implanted in athymic nude mice. Representative photographs of harvested tumors from control and glibenclamide-treated Capan-1 cells (A) and SU.86.86 cells (E). Tumor growth curves of Capan-1 (B) and SU.86.86 (F) tumor-bearing nude mice treated with vehicle or glibenclamide. Tumor weights between control and glibenclamide-treated Capan-1 (C) and SU.86.86 cells (G). Evaluation of mouse body weights during the xenograft experiment in Capan-1 (D) and SU.86.86 (H). Subcutaneous xenograft of control SU.86.86 cells and SU.86.86/shRNA-PEPT1 in athymic nude mice showing representative images of harvested tumors (I), tumor growth curves (J), tumor weight (K), and mice body weight (L) between control and PEPT1-knockdown groups. Data are given as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.