Figure 6.
![(A) Real-time PCR showing relative PEPT1 mRNA expression in control SU.86.86 cell line vs. the SU.86.86/shRNA-PEPT1 cell line. (B) Western blotting showing PEPT1 protein expression in control SU.86.86 cell line vs. the SU.86.86/shRNA-PEPT1 cell line. β-Actin was used as the endogenous control. (C) PEPT1-mediated [3H]-Gly-Sar uptake in control SU.86.86 cell line vs. the SU.86.86/shRNA-PEPT1 cell line. (D) Clonogenic assay in control SU.86.86 cell line vs. the SU.86.86/shRNA-PEPT1 cell line. Data are given as mean ± SEM. *** P < 0.001, **** P < 0.0001.](https://port.silverchair-cdn.com/port/content_public/journal/biochemj/478/20/10.1042_bcj20210377/2/bcj-2021-0377.06.png?Expires=1716923712&Signature=Oj3rkXOlvhDuOOsMW3PqguMju3edTZlLHGklwqHk74PgipunXlLtkh7jGNSuAOdedzpFQsPKoiSd881aRVjV7UDnA4a9JUu5KITogCzjNNG2vVJF1lZaYGrMQ52VhsxpDPo7EgCV46nmFUU87nb~qbzovRWe3CS9Au36eQZxrLHX6hAm8zX8FmmxmQZ0Ax36sF8~e9dw3TWObBRf8NEsxZT9Ap-le8hvSo9TLP0uVN071hVatj9AELTcbw1F9~ANpWasY0ZbQTUjHtfP9XABaRhUuVepkdPI6Ah5x7cAweb9dxjg1Dfkeo2CLHONuHRj7~VT4cMESkFSN~DscK053Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Genetic knockdown of PEPT1 reduces colony formation ability of PDAC cells.
(A) Real-time PCR showing relative PEPT1 mRNA expression in control SU.86.86 cell line vs. the SU.86.86/shRNA-PEPT1 cell line. (B) Western blotting showing PEPT1 protein expression in control SU.86.86 cell line vs. the SU.86.86/shRNA-PEPT1 cell line. β-Actin was used as the endogenous control. (C) PEPT1-mediated [3H]-Gly-Sar uptake in control SU.86.86 cell line vs. the SU.86.86/shRNA-PEPT1 cell line. (D) Clonogenic assay in control SU.86.86 cell line vs. the SU.86.86/shRNA-PEPT1 cell line. Data are given as mean ± SEM. *** P < 0.001, **** P < 0.0001.