CCT3 is critical for cell survival and invasion of HNSCC cells
(A) CERES score was obtained from Depmap datasource and used to access the significance of CCT3 for cell survival of HNSCC. CERES score approach to -1 means the gene is important for cell survival, while score approach to 0 means the gene is not an essential gene. (B) SCC25 and CAL27 cells were transfected with control siRNA or siRNA-CCT3 for 48 h. Cells were harvested and subjected to Western blot assay to detect the expression of CCT3. A CCK-8 assay was performed to detect cell viability of SCC25 (C) and CAL27 (D) cells after being transfected with siRNA. (E) A wound healing assay was performed to determine the cell invasion of SCC25 and CAL27 cells after being transfected with siRNA. The cells were photographed at 0 or 48 h; *, P < 0.05; **, P < 0.01; ***, P < 0.001; CCT3, Chaperonin-containing TCP-1 3.