EM, SMLM and STED images visualising astrocyte processes in the vicinity of excitatory synapses
(A) EM image of diaminobenzidine-labelled EGFP/RPL10A (arrowheads) in astrocyte processes (green) near cortical synapses (axon = red and PSD = blue); scale bars = 500 nm; modified from [106] with permission. (B) SMLM image showing an EGFP/RPL10A (green) filled astrocyte process near a synapse (presynaptic bassoon (red) and postsynaptic Homer (blue); inset of box on left, and side view is a 90° rotation of a second synapse; modified from [106] with permission. (C) Deconvoluted confocal microscopy image of an astrocyte (hGfap-eGFP, green), presynaptic vGluT1 (blue) and postsynaptic Homer1 (red); the magnified area shows the STED image for vGluT1 and Homer1 merged with deconvoluted confocal image for eGFP; scale bar = 10 µm; modified from [107] with permission.