Digoxin induced G1 and G2/M phase arrest in A549 and H1299 cells, respectively
(A) A549 and H1299 cells were incubated with various concentrations of digoxin for 24 h, then the cell cycle distribution was determined by flow cytometry. (B) The percentages of the cell population in G1, S, and G2/M phases. (C) Western blotting analysis of the cell cycle-related proteins in cellular extracts from A549 and H1299 cells treated with various concentrations of digoxin for 24 h. The data are expressed as mean ± SD (n=3); ∗P<0.05, ∗∗P<0.01, ∗∗∗P<0.01, compared with control.