(A) Representative images of positive and negative CDKN2A protein expression of hepatocellular carcinoma tissues. CDKN2A was expressed in cytoplasm (yellow arrow) and nucleus (red arrow) of tumor cells. There were 35 patients stained positive and 35 patients stained negative. The scales bar to indicate 20 μm. (B) Kaplan–Meier analysis of CDKN2A protein in hepatocellular carcinoma patients. Patients with higher CDKN2A expression had shorter OS compared with patients with lower CDKN2A expression (P=0.0053, HR = 1.64). (C) Univariate and multivariate cox regression showed that higher CDKN2A protein expression and advanced pathological stage were independent prognostic factor in hepatocellular carcinoma patients. black: low mRNA expression.