Interactions of amino acid residues with the substrate of MAM synthase from Brassica juncea
(A) 3D format of the substrate 4-methyl-thio-2-oxobutanoic acid. (B) Interactions of the encircled amino acid residues (V182, E233, A253 and P255) with the substrate 4-methyl-thio-2-oxobutanoic acid (labelled as KMT603) and Mn2+ and other amino acid residues around the active site of BjuMAM1.1 (PDB ID: 6E1J, MAM synthase from Brassica juncea) [4]. The bonds are represented by different colours: hydrogen bonds (blue), hydrophobic contacts (grey) and metal interactions (purple), respectively.