(A) H292 cells were transfected with empty vector (EV) or USP52-HA plasmids for 48 h, followed by Western blot against p-AKT, AKT, p-mTOR, mTOR and HA. GAPDH was used as a loading control. (B) The relative abundance of p-AKT and p-mTOR in H292 from (A) were quantified by gray scanning after USP52-HA plasmids transformed at 0, 1, 2, 4 μg. (C) H460 cells were transfected with empty vector (EV) or USP52-HA plasmids for 48 h, followed by Western blot against p-AKT, AKT, p-mTOR, mTOR and HA. GAPDH was used as a loading control. (D) The relative abundance of p-AKT and p-mTOR in H460 from (C) were quantified by gray scanning after USP52-HA plasmids transformed at 0, 1, 2, 4 μg. These experiments were repeated for three times. Groupt p-AKT: One-way ANOVA, *P<0.05, **P<0.01. Group p-mTOR: One-way ANOVA, ^#P<0.05, ^##P<0.01. NS, not significant.