SRA expression and regulation of cell proliferation in ovarian cancer cell lines
(A) The expression of SRA was significantly higher in some ovarian cancer cell lines (OVCA429, TOV112D, SKOV3, A2780, OVCA433) than in normal control cells (HOSE). *P<0.05, **P<0.01 vs. siNC. (B) OVCA429 cells were transfected with SRA-specific siRNA and negative-control siRNA and knockdown efficiency was determined by qRT-PCR analysis. qRT-PCR was performed in triplicate. ***P<0.001 vs. siNC. (C) The proliferation of OVCA429 cells transfected with siSRA and negative control siRNA was determined using the CCK-8 assay. Bars indicate mean ± SD of three independent experiments. *P<0.05 vs. siNC. (D) Overexpression of SRA in OVCA433 cells was determined by qRT-PCR analysis. qRT-PCR was performed triplicate. *P<0.05 vs. vector. (E) Cell proliferation was analyzed using CCK-8 assays. Bars indicate the mean ± SD of three independent experiments. *P<0.05, **P<0.01 vs. OVCA433, Vector cells. Abbreviation: SD, standard deviation.