(Left) MSP nanodisc solubilisation — MP is initially extracted into detergent micelles to which lipid and MSP are added before detergent removal triggers nanodisc formation. (Right) Amphipathic polymer nanodisc solubilisation — addition of amphipathic polymer to the membrane fraction results in the spontaneous formation of nanodiscs encapsulating the native lipid and protein. Figure created with BioRender.com.