Figure 3
(A,B) ALP levels detected by ALP staining and activity assay showed that the concentration of PTH at which the osteogenic differentiation of HBMSCs was the strongest was 10−9 M. (C) Matrix mineralization determined by Alizarin Red staining also indicated that 10−9 M PTH was the best for osteogenic differentiation in LPS-induced HBMSCs. Data are mean ± SD. *P<0.05 and **P<0.01 versus the control (HBMSCs) group; ##P<0.01 versus the LPS (HBMSCs + LPS) group, n=5. Scale bar, 200 μm.
A concentration of 10−9 M as the optimal PTH level on osteogenic differentiation in LPS-induced HBMSCs

(A,B) ALP levels detected by ALP staining and activity assay showed that the concentration of PTH at which the osteogenic differentiation of HBMSCs was the strongest was 10−9 M. (C) Matrix mineralization determined by Alizarin Red staining also indicated that 10−9 M PTH was the best for osteogenic differentiation in LPS-induced HBMSCs. Data are mean ± SD. *P<0.05 and **P<0.01 versus the control (HBMSCs) group; ##P<0.01 versus the LPS (HBMSCs + LPS) group, n=5. Scale bar, 200 μm.

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