Tau secretion is reduced by tau-MBD and tau-C291/322A mutations in N2A cells
(A) A representative dot blot image of cell lysate and conditioned media from N2A cells transfected with wildtype tau, tau-MBD mutant or tau-C291/322A mutant, stained with tau-5 antibody. (B) Quantification of the densitometric dot blot data (n=6). Conditioned media dots were normalised by the corresponding cell lysate dots. Data are represented as mean ± SEM. **P<0.01, ****P<0.0001 (Welch’s ANOVA). (C) Confocal micrographs of N2A cells transfected with wildtype or mutant tau (tau-MBD or tau-C291/322A). Representative images of tau-5 immunostaining with Hoechst 33342 nuclear counterstain are shown. Scale bar, 10 µm. (D) Western blot analysis of N2A cells transfected with wildtype or mutant tau (tau-MBD or tau-C291/322A), stained with tau-5 antibody. β-actin was used as a loading control.