Many systems and synthetic biology tools and approaches, for example CRISPR/Cas9-based gene editing, omics profiling, parts mining, static and dynamic optimization methods, have been developed for Halomonas spp. It is thus advanced, the genetic reprogramming of Halomonas spp. allowing construction of high-performance Halomonas cell factories for production of a variety of chemicals, polyesters and proteins. A cost-effective NGIB has been developed based on extremophilic bacteria especially Halomonas spp. for bioproduction in various scales. Abbreviation: CRISPR, clustered regularly interspaced short palindromic repeats.