Genes encoding for proteins identified as Akirin2 IPs and differentially regulated in response to akirin2 KO were selected to validate transcriptomics data by qRT-PCR. (A) Heatmap of RNAseq results for selected genes. KO1-KO3 and WT1-WT3 mean values are shown. Heatmap was prepared with average linkage and Euclidean distance measurement method. (B) Normalized mRNA levels (average + S.D. Ct values) were compared between WT and KO cells by Student’s t test (P<0.05; n=3 biological replicates). (C) WT to KO ratio of normalized mRNA levels. (D) Western blot analysis of total protein extracts from WT and akirin2 KO HL60 cells. One microgram of recAkirin2-tagged protein produced in human cells was used as positive control. Akirin2-specific primary antibodies were used for Akirin2 detection. For quantitation, gels were scanned with ImageJ (https://imagej.nih.gov/ij/index.html).