The effect of akirin2 KO was evaluated on the transcriptome and proteome of human HL60 cells. Results of differential gene expression and protein representation in akirin2 KO and WT cells and analysis of GO annotations for BPs were used to characterize Akirin2 gene/protein targets and to identify Akirin2 functional complements. The BPs that are not related to known Akirin2 IPs and thus associated only with Akirin2 were identified and selected for further analysis (green arrows). Genes in these BPs may be regulated by Akirin2 through unknown IPs, indirect (genetic) interactions or direct chromatin remodeling. To address the possibility of direct chromatin remodeling, Akirin2–histone physical interactions were then investigated.