(A) Representative H&E staining (upper) and Oil Red O staining (lower) of liver sections (n = 5–8/group, three images/mouse); scale bars: 100 µm. (B) Liver weights. (C) Liver weight-to-body weight ratios. (D) Liver and (E) serum triglyceride content. (F) Liver and (G) serum cholesterol content. (H) Serum LDL and (I) HDL. (J) Cumulative food intake. (K) Serum ALT and (L) AST levels. (M) The ability of fatty acid uptake in HepG2 cells pre-treated with GUDCA or vehicle for 24 h was measured fluorometrically by using BODIPY-C16. (N) Fluorescence images of BODIPY -C16 (green) were acquired (400×; n = 5–8/group). All data are presented as the mean ± SEM and analyzed by one-way ANOVA followed by the Bonferroni post hoc test. *P<0.05, **P<0.01 GUDCA versus vehicle; ^#P<0.05, ^##P<0.01 TUDCA versus vehicle.