HFD-fed mice were treated with vehicle or GUDCA (80 mg/kg/d) or TUDCA (350 mg/kg/d) during the last 3 weeks (n = 5–8/group). (A) Bile acid profiles in the serum after 3 weeks GUDCA or TUDCA treatment. (B) Body weight changes after administration. (C) GTT, (D) AUC, (E) ITT, (F) kITT value, (G) fasting insulin levels and (H) fasting glucose levels. (I) Irs1 and Akt signaling in liver. Data are presented as mean ± SEM and analyzed by one-way ANOVA followed by the Bonferroni post hoc test; *P<0.05, **P<0.01, ***P<0.001 GUDCA versus vehicle; ^#P<0.05, ^##P<0.01, ^###P<0.01 TUDCA versus vehicle.