Thirty micrograms of total cell lysates from each Calu-3 subline was subjected to Western blot analysis. (A) Reduced PKCα expression is seen only in samples overexpressing the wildtype membrane-anchored prostasin (lane 5). (B–D) Results of four independent experiments were analyzed, quantified and presented as bar graphs: total PKCα normalized against GAPDH (B); phosphorylated PKCα normalized against GAPDH (C); ratio of phosphorylated PKCα in total PKCα content (D). Data are presented as mean ± SD, * denotes P<0.05, as compared with the controls (CC or V).