(A) Representative images of Western blot analysis on Calu-3 sublines treated with 100 ng/ml IFNγ for 30 min. Calu-3P cells without the treatment (0 h) and with the treatment for 48 h were used for monitoring protein phosphorylation as indicated. Both pStat1Tyr⁷⁰¹ and pStat1Ser⁷²⁷ were increased upon IFNγ treatment, while phosphorylation of Stat1Ser⁷²⁷ sustained for at least 48 h. (B–D) Bar graphs of phosphorylated proteins in (A), * denotes P<0.05, as compared with the vector control (V).