After overnight serum starvation in RPMI medium with 0.5 mM glucose, RAW 264.7 cells were treated with 20 mM glucose in the presence or absence of azaserine for 4 h. (A) Cell lysates were subjected to the Western blot analysis using α-E catenin and α-Tubulin antibodies. (B) Densitometry analysis showing protein expression of α-E catenin normalised to α-Tubulin. Data represent mean ± S.E.M of three independent experiments. The unpaired t test was used to assess statistical significance, *P<0.05. RAW 264.7 cells were treated with 20 mM glucose in the presence or absence of tunicamycin (10 μg/ml) for 2 h. (C) Cell lysates were subjected to the Western blot analysis using α-E catenin and α-Tubulin antibodies. (D) Densitometry analysis showing protein expression of α-E catenin normalised to α-Tubulin. Data represent mean ± S.E.M of four independent experiments. The unpaired t test was used to assess statistical significance, *P<0.05.