The muscarinic agonist carbachol promotes the shedding of klotho through activation of ADAM10
SH-SY5Y cells stably expressing klotho were incubated for 24 h with the muscarinic agonist carbachol (10 µM) and the selective ADAM10 inhibitor GI254023X (20 µM) as indicated. (A) Lysates and media were then prepared and immunoblotted for klotho with ab154163. (B) Quantitation of the amount of klotho in the media with the untreated cells set to 100%. Data are presented as mean ± SEM, n=4, Mann–Whitney test. * = P<0.05.