Cardiomyocytes were uninfected, or infected with adenoviruses containing constructs for WT-MAP4K4, MAP4K4(T187A) or MAP4K4(T187D). Serum was withdrawn and cells were incubated for 48 h in serum-free medium. (A) Cells were immunostained with antibodies to the FLAG epitope (right panels, green) or troponin T (left panels, red). (B) Cardiomyocyte surface area was measured using ImageJ. Results are shown for four independent cardiomyocyte preparations. Mean values for each experiment (calculated from a minimum of 50 cells per condition) are plotted, together with the overall means ± S.E.M.