C57Bl/6J male mice were treated with vehicle alone (Control), or with phenylephrine (PE; 40 mg/kg/d) with/without linsitinib (2 mg/kg/d) using osmotic minipumps. Cardiac function/dimensions were assessed by echocardiography. (A) Representative M-mode images at baseline and 4 days post minipump implantation. Quantitative assessment of cardiac function (B) and left ventricular cardiac dimensions in diastole (C) and systole (D) at baseline (BL) and 4 days. WT:ID, ratio of wall thickness (WT = anterior wall + posterior wall) to internal diameter (ID). Individual data points are shown with means ± SEM (n = 7 per group). Statistical analysis used two-way ANOVA with Holm–Sidak's post-test.