Effects of IGF2 knockout on mitochondrial biogenesis in skeletal muscle cells
(A) Mitochondrial mass was measured using MitoTracker staining. Scale bar = 100 μm. (B) Relative mtDNA contents after normalization to nuclear DNA (nDNA) levels. MT1–3 represents three primers mapping to distinct regions on mtDNA. (C) mtDNA transcription levels were measured by qRT-PCR. Data represent mean ± SD of three biological replicates. *P<0.05, **P<0.01, ***P<0.001, n.s., not significant.