Figure 4
(A) Protein abundance of mitochondrial subunits complex I (NDUFB8), complex II (SDHB), complex III (UQCRC2), complex IV (MTCO1), complex V (ATP5A) in siNC and siIGF2 skeletal muscle cells. (B) Effects of IGF2 knockout on the content of proteins regulating mitochondrial fusion (MFN) and fission (DRP) in siNC and siIGF2 skeletal muscle cells measured by western blot. Data represent mean ± SD of three biological replicates. *P<0.05, **P<0.01, ***P<0.001, n.s., not significant.
Changes in markers of mitochondrial protein content in IGF2 knockdown group compared with control group

(A) Protein abundance of mitochondrial subunits complex I (NDUFB8), complex II (SDHB), complex III (UQCRC2), complex IV (MTCO1), complex V (ATP5A) in siNC and siIGF2 skeletal muscle cells. (B) Effects of IGF2 knockout on the content of proteins regulating mitochondrial fusion (MFN) and fission (DRP) in siNC and siIGF2 skeletal muscle cells measured by western blot. Data represent mean ± SD of three biological replicates. *P<0.05, **P<0.01, ***P<0.001, n.s., not significant.

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