Figure 2.
![(a) Free copper reactions based on [44] (b) Cu-LPMO reactions based on [31,48]. Pathway I: O2 binds to the Cu(I)-active site of the Enzyme (E). In the presence of a polysaccharide substrate (S), O2 reduction leads to substrate hydroxylation. In the absence of a polysaccharide, O2 reduction produces H2O2. Pathway II: H2O2 generated in situ can react with the Cu(I)-active site. When the polysaccharide is bound, substrate oxidation occurs. Small-molecule reductants or enzymes, such as cellobiose dehydrogenase, can provide the necessary reducing equivalents.](https://port.silverchair-cdn.com/port/content_public/journal/biochemsoctrans/49/1/10.1042_bst20201031/1/bst-2020-1031c.02.png?Expires=1717006990&Signature=MqLWbwjWoBYkZLX1Uh4SMbiTWLkBh0NF~CLMhyLvMmdqdW8S5xlws3HrjDvP4zr1XSLkeou4Clhnt8AeVIBm8eAS1aVx8vxwJZkuvRBZKikgz9xkUhPpPYzoOOdM~-~d4el4RNuQO5KfWvxUJV2KeY2Lwb-SmzRbaarSJ6j5vX7HVS4qsw1Bsdkm9m7jTukzwgn6lHx21E2iGxWXAa7wV-euRvU3H296Km6AUnRY0xiuTWENa8Wn-foyhCvAsltm1Ftq5p8ghzU9Ceacr1Fk2MrR-NqJv83r5US744RRUYY6Zeuy0ZFGHXc39ubd1j53lV48eClUaJavdsSggdP7AA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Simplified reaction schemes for free copper and Cu-LPMO.
(a) Free copper reactions based on [44] (b) Cu-LPMO reactions based on [31,48]. Pathway I: O2 binds to the Cu(I)-active site of the Enzyme (E). In the presence of a polysaccharide substrate (S), O2 reduction leads to substrate hydroxylation. In the absence of a polysaccharide, O2 reduction produces H2O2. Pathway II: H2O2 generated in situ can react with the Cu(I)-active site. When the polysaccharide is bound, substrate oxidation occurs. Small-molecule reductants or enzymes, such as cellobiose dehydrogenase, can provide the necessary reducing equivalents.