(A,B) Total quantity of (A) PI and (B) LPI in mouse cerebra. (C) Pie charts of the PIs identified in mouse cerebra. (D,E) The quantity of LPI 20:4 specified by the digested positions. (D) LPI 20:4 (sn-1) catalyzed by PLA₂ and (E) LPI 20:4 (sn-2) catalyzed by PLA₁. Ddhd1(+/−) and Ddhd1(−/−) are shown in opened and filled columns, respectively. The unit of the vertical axis is pmol/mg. Error bars represent the mean ± SD. For Ddhd1(+/−) (n=2) versus Ddhd1(−/−) (n=2), *: P<0.05. N.S., not significant. All data were analyzed using a two-tailed Student’s t test.