Selected clones show lower HTRA1 expression than parental SK-N-SH cells
SK-N-SH cells and selected clones 1–4 were cultured with 10 µM ATRA or DMSO control for 6 days. The mRNA expression of HTRA1 was normalised to the housekeeper genes, GAPDH and HPRT1, and SK-N-SH DMSO expression was used as 1 for reference. Data are derived from one representative experiment performed in triplicate. Individual data points are shown. Histogram bars represent mean values and error bars represent S.E.M. Graph shows *P<0.05 and ***P<0.001, compared with each DMSO condition by two-tailed t test; ###P<0.001 compared with the SK-N-SH DMSO condition and †††P<0.001 compared with the SK-N-SH ATRA condition by one-way ANOVA with Dunnett’s post-hoc analysis.