(A) HOPS (red) nucleo-cytoplasmic shuttling was observed after EGF treatment. Nuclei were DAPI stained (blue). Bars, 5 μm. (B–D) HOPS localisation (red) in differentiating lens primary cell cultures mimicking in vivo development. HOPS immunolocalisation was analysed in lentoid (B), proliferating (C) and differentiating cultured cells (D). Nuclei were DAPI stained (blue). γ-tubulin (green) was used as marker. Bars, 200 μm in (B) and 5 μm in (C,D). Merged images are reported. Representative images are shown.