(A,B) Mouse eye (A) and lens (B) schematic overview with main components indication. (C) WB analysis of HOPS protein expression in eye and lens compartments (left). The fibers proteins of lens cortex and nucleus obtained upon Tris/HCl (lanes 1, 2) and urea treatment (lanes 3, 4) evaluated by WB (right). Ponceau S staining was used to evaluate protein amount. Images are representative of one mouse eye proteins. (D) qPCR showing Hops-mRNA in lens epithelium, cortex and nucleus. mRNA in lens epithelium was assumed as 1 and samples were normalised to Gusb gene. Representative images are shown.