MicroRNA (MiR)-141 is the direct target of small nucleolar RNA host gene 15 (SNHG15)
(A) The predicted complementary binding site of SNHG15 and miR-141. (B) The luciferase activity in human glomerular mesangial cells (HGMCs) co-transfected with pGL3-SNHG15 WT/pGL3-SNHG15 MUT and miR-141 mimics/NC was determined by dual luciferase reporter (DLR) assay. ***P<0.001 vs. the miR-negative control (NC) group. (C) The expression of miR-141 in diabetic nephropathy (DN) tissues (n=15) and normal tissues (n=20) was detected by quantitative reverse-transcription PCR (qRT-PCR). ***P<0.001 vs. normal. (D) The correlation between SNHG15 and miR-141; P<0.0001, R2 = 0.701. (E) The expression of miR-141 after transfection of shRNA (sh)-SNHG15/NC or overexpression (oe)-SNHG15/pcDNA3.1 into high-glucose (HG)-induced human glomerular mesangial cells (HGMCs) was detected by qRT-PCR. ***P<0.001 vs. the sh-NC group; ### P<0.001 vs. the pcDNA3.1 group.