Figure 1
Release of inflammatory factors in high-glucose (HG)-induced human glomerular mesangial cells (HGMCs) is restrained by small nucleolar RNA host gene 15 (SNHG15) knockdown
(A) The expression of SNHG15 in diabetic nephropathy (DN) tissues (n=15) and normal tissues (n=20) was detected by quantitative reverse-transcription PCR (qRT-PCR); ***P<0.001 vs. normal. (B) The expression of SNHG15 in HG-stimulated HGMCs was determined by qRT-PCR; ***P<0.001 vs. the normal glucose (NG) group. (C) The expression of SNHG15 after transfection of shRNA (sh)-SNHG15/negative control (NC) or overexpression (oe)-SNHG15/pcDNA3.1 into HG-stimulated HGMCs was detected by qRT-PCR. (D) The viability of HG-stimulated HGMCs was measured by MTT assay. (E) The levels of IL-1β, IL-6 and TNF-α in HG-stimulated HGMCs were measured by ELISA. ***P<0.001 vs. the sh-NC group; ### P<0.001 vs. the pcDNA3.1 group.