Asm inhibition reduces vascular calcification during cholecalciferol overload in mice
(A) Scatter dot plots and arithmetic means ± SEM (n=8; µg/mg protein) of calcium content in the aortic arch of mice receiving vehicle (CTR) or high-dosed cholecalciferol (vD) without or with additional treatment with amitriptyline (Ami) or fendiline (Fen). (B–E) Scatter dot plots and arithmetic means ± SEM (n=5; arbitrary units, a.u.) of Msx2 (B), Cbfa1 (C), Alpl (D) and Sgk1 (E) relative mRNA expression in aortic tissue of mice receiving vehicle (CTR) or high-dosed cholecalciferol (vD) without or with additional treatment with amitriptyline (Ami) or fendiline (Fen). *(P<0.05), **(P<0.01), ***(P<0.001) significant compared with CTR mice; †(P<0.05), ††(P<0.01), †††(P<0.001) significant compared with vD-treated mice.