Figure 2
(A) Representative confocal microscopy images showing ceramide levels in HAoSMCs following treatment for 5 min with control (CTR), bacterial sphingomyelinase (SMase) or β-glycerophosphate (Pi) alone and together with bacterial sphingomyelinase (SMase). Ceramide: green labeling, nuclei: magenta labeling. Scale bar: 50 μm. (B) Representative histograms of flow cytometry analysis and scatter dot plots and arithmetic means ± SEM (n=6; arbitrary units, a.u.) of normalized ceramide-dependent fluorescence intensity in HAoSMCs following treatment for 5 min with control (CTR), bacterial sphingomyelinase (SMase) or β-glycerophosphate (Pi) alone and together with bacterial sphingomyelinase (SMase) or amitriptyline (Ami). **(P<0.01), ***(P<0.001) significant compared with control HAoSMCs; †††(P<0.001) significant compared with Pi-treated HAoSMCs.
Phosphate increases ceramide levels in HAoSMCs

(A) Representative confocal microscopy images showing ceramide levels in HAoSMCs following treatment for 5 min with control (CTR), bacterial sphingomyelinase (SMase) or β-glycerophosphate (Pi) alone and together with bacterial sphingomyelinase (SMase). Ceramide: green labeling, nuclei: magenta labeling. Scale bar: 50 μm. (B) Representative histograms of flow cytometry analysis and scatter dot plots and arithmetic means ± SEM (n=6; arbitrary units, a.u.) of normalized ceramide-dependent fluorescence intensity in HAoSMCs following treatment for 5 min with control (CTR), bacterial sphingomyelinase (SMase) or β-glycerophosphate (Pi) alone and together with bacterial sphingomyelinase (SMase) or amitriptyline (Ami). **(P<0.01), ***(P<0.001) significant compared with control HAoSMCs; †††(P<0.001) significant compared with Pi-treated HAoSMCs.

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