The best fit (Fit #3, $χ2¯=0.47$⁠) with n₁ = 1, n₂ = 1 is shown with solid lines, next best fit with n₁ = 1, n₂ = 2 (Fit #6, $χ2¯=1.73$⁠) is shown with dashed lines, fit with variable n₁ and n₂ (Fit #9, $χ2¯=0.49$⁠) is not shown, since it overlaps with the best fit #3:

(A) concentrations of the protein species: free FVIIa (red), FVIIa with a C6PE bound to the first site (green), FVIIa with a C6PE bound to the second site (black), FVIIa with both C6PE sites occupied (blue); (B) FVIIa proteolytic activity; (C) FVIIa intrinsic tryptophan fluorescence Intensity; (D) FVIIa amidolytic activity; (E) concentration of bound lipid (ΔP) as determined by equilibrium dialysis.