Omeprazole pretreatment inhibited chemotherapy-induced autophagy and improve the chemosensitivity of GC cells
(A) MTT assay was performed to evaluate the relative survival rate of AGS and HGC-27 cells treated with three kinds of different antitumor drugs with or without omeprazole preconditioning. *P<0.05 versus cells in corresponding groups; **P<0.01 versus cells in corresponding groups (Con: cells without any special treatments). (B) Annexin V-FITC/propidium iodide (PI) staining assay was used to detect apoptosis of GC cells. (C) Western blot assay was used to estimate the protein expression levels of autophagy-related markers in antitumor drug-treated GC cells (Con: cells without any special treatments). (D) Western blot assay was used to estimate the protein expression levels of autophagy-related markers in antitumor drug-treated GC cells with or without omeprazole preconditioning. (E) LC3-GFP (in AGS) or LC3-RFP (in HGC-27) puncta was used to analyze the autophagy condition of lung cancer cells treated as indicated. The representative images of GC cells treated with antitumor drugs with or without omeprazole were shown. The magnification was 400×; meanwhile, the number of puncta per cell was counted (F); **P<0.01 versus corresponding NC groups.