(A) HUVECs cytoskeleton F-actin was stained with phalloidin after miR-1-3p NC/mimics/inhibitor transfection with or without LPS treatment. The images are representative of three experiments with similar results. (B–E) Western blotting analysis of p-MLC, p-FAK397, and Cx43 expression. β-actin served as an internal reference. The blots are representative of at least three independent experiments with similar results. (F) Transwell assays measured the effect of miR-1-3p on monolayer cell permeability by detecting the absorbance of BSA-Evans Blue (n=3, from three independent experiments). (G) LDH activity detection in the supernatant of transfected cells by an LDH detection kit (n=3, from three independent experiments). Data are presented as mean ± SE, *P<0.05.