(A) HUVECs were treated with LPS at different concentrations (0.1, 1, 5, 10, and 100 μg/ml). Cytoskeleton F-actin was stained with phalloidin and observed under a fluorescence microscope (400×). (B) MiR-1-3p expression analysis of LPS (10 μg/ml) stimulated HUVECs for 24 h by RT-PCR (n=4, from four independent experiments). (C) MiR-1-3p expression of HUVECs transfected with miR-1-3p mimics, inhibitor, and NC by RT-PCR detection (n=4, from four independent experiments). (D,E) CCK-8 for cell proliferation tests with or without LPS stimulation (n=6, from three independent experiments with duplicate, *P<0.05 in NC vs mimics, **P<0.05 in NC vs inhibitor, ***P<0.05 in mimics vs inhibitor). (F,G) Cell apoptosis assay by flow cytometry (n=3, from three independent experiments). (H–N) Western blotting evaluated the expression of apoptosis-related proteins active-casp3, Bcl-2, Bax, ER stress protein GRP78, VEGF, and proinflammatory cytokines IL-1β and iNOS. β-actin served as an internal reference. The blots are representative of at least three independent experiments with similar results. Data are presented as mean ± SE, *P<0.05.