Effects of different culture media on the activation of primary HSCs
Quiescent HSCs cultured under different test media (standard, 1–5) were examined for activation by LD content using ORO dye staining at day 3 (A–D) and day 7 (E–H); by immunofluorescent staining for α-SMA day 3, (N–Q) and day 7 (I,K–M). Quantification of LDs accumulation at day 3 (R), and day 7 (S) were shown. mRNA levels of the activation-related genes (a-SMA, COL I) and quiescent-related gene (LRAT) at day 3 (T), and day 7 (X). Data are shown as means ± SEM, n=3, *P<0.05. The arrowheads show the ORO-stained area. Scale bar: 50 μm were not noted. Abbreviation: COL I, type I collagen.